science, biology, chemistry, biochemistry, deuterium, heavy water, stable isotops, biosynthesis, metabolism, molecular evolution, adaptation
Russian scientist, biochemist, researcher of water, engineer of technology (1992), candidate of Science (Ph. D in Chemistry) Dr. Oleg V. Mosin was bourn in a military family and spent much time in Ural region. In 1996 he graduated post-graduate studies from Moscow State Academy named after M.V. Lomonosov on biotechnological methods of stable isotope labeled compounds and further worked under the leadership of prominent Soviet and Russian scientist the founder of Soviet and Russian school of biotechnology Doctor of Sciences, academician, professor Vitaly I. Shvetz where he studied many living objects to resistance to highly deuterium content in growth media. His dissertation thesis was entitled of “The developing of various biotechnological methods for the preparation of proteins, amino acids and nucleosides labeled with stable isotopes - 2H and 13C with highly levels of isotopic enrichment”. In the frames of his work many living cells of different origin were adapted to high deuterium content in growth media by employing a special adaptation technique. The scope of his scientific interests comprising water (heavy water), and its structure, the questions of keeping an information by water, stable isotopes, application of various microbial objects for the preparation of cell compounds labelled with stable isotopes 2H, 13C, 15N, including proteins, amino acids and nucleosises; biosynthesis and metabolism of isotopically labeled compounds; biological adaptation to heavy water, effects of heavy water on living objects; and molecular evolution. His research deals with preparation of isotopically labelled cell compounds with using microbial strains; biological adaptation to heavy water; preparation of [2H]-and [13C]-amino acids with high levels of enrichment via bioconvertion of methanol/13C-methanol/deuteron-methanol and heavy water in methylotrophs Brevibacterium methylicum and Methylobacillus flagellatum; the studying of L-Phe biosynthesis by facultative methylotroph Brevibacterium methylicum in the presence of heavy water; biosynthesis of bacteriorhodopsin labeled with deuterium on the residues of aromatic amino acids [2,3,4,5,6-D5]phenylalanine, [3,5-D2]tyrosine and [2,4,5,6,7-D5]tryptophan; the growth of Bacillus subtilis and biosynthesis of inosine on highly deuterated media, containing heavy water and deuterated biomass of methylotrophic bacterium B. methylicum; electron impact mass-spectrometry and NMR in evaluation of levels of enrichment in labelled cell compounds, and cell adaptation to heavy water. Dr. Oleg V. Mosin is a winner of the President Prize (1996) for distinguished successes in studies and scientific research and the Prize of Prof. Marinov (Boulgaria, 2010).
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